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cd14 microbeads miltenyi biotec  (Miltenyi Biotec)


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    Miltenyi Biotec cd14 microbeads miltenyi biotec
    Cd14 Microbeads Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+cell+isolation+kit/CD1c+(BDCA-1)%2B+Dendritic+Cell+Isolation+Kit%2C+human/10__1016_slash_j__isci__2026__116736-639-174-176
    Average 94 stars, based on 23 article reviews
    cd14 microbeads miltenyi biotec - by Bioz Stars, 2026-10
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    Related Articles

    Isolation:

    Article Title: M3 muscarinic acetylcholine receptor–reactive Th17 cells in primary Sjögren’s syndrome
    Article Snippet: .. First, CD14 + monocytes were isolated from PBMCs by positive selection using CD14 cell isolation kit (catalog 130-050-201, Miltenyi Biotec). ..

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Selection:

    Article Title: M3 muscarinic acetylcholine receptor–reactive Th17 cells in primary Sjögren’s syndrome
    Article Snippet: .. First, CD14 + monocytes were isolated from PBMCs by positive selection using CD14 cell isolation kit (catalog 130-050-201, Miltenyi Biotec). ..

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy
    Article Snippet: ESCs or DSCs were cultured in DMEM/F-12 (HyClone, SH30023.01) containing 10% FBS (Gibco, 26140-079). .. DMφ from DICs were obtained through positive selection by an CD14 + cell isolation kit according to the reagent instructions (Miltenyi Biotec, 130–050-201). ..

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Cell Isolation:

    Article Title: M3 muscarinic acetylcholine receptor–reactive Th17 cells in primary Sjögren’s syndrome
    Article Snippet: .. First, CD14 + monocytes were isolated from PBMCs by positive selection using CD14 cell isolation kit (catalog 130-050-201, Miltenyi Biotec). ..

    Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
    Article Snippet: THP-1 monocytes (ATCC: TIB-202) were differentiated into macrophages using 100 nM PMA. .. For the generation of human MDM, peripheral blood CD14 + cells were positively selected by MACS using CD14 + cell isolation kit (Miltenyi Biotech, Bergisch Gladbach, Germany) according to manufacturer’s instructions and differentiated with GM-CSF at 20 ng/ml for 7 days in RPMI 1640. ..

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy
    Article Snippet: ESCs or DSCs were cultured in DMEM/F-12 (HyClone, SH30023.01) containing 10% FBS (Gibco, 26140-079). .. DMφ from DICs were obtained through positive selection by an CD14 + cell isolation kit according to the reagent instructions (Miltenyi Biotec, 130–050-201). ..

    Article Title: Single-cell transcriptomics reveals prominent expression of IL-14, IL-18, and IL-32 in psoriasis.
    Article Snippet: In a final step, percentages for negative and 1+ to 3+ positive cells based on these representative slides were illustrated using RStudio (version 2022.07.2+576). .. Human DC were generated from CD14-positive monocytes from whole blood using a CD14+ cell isolation kit (Miltenyi) as described [50]. .. Cells were cultured in DC-medium (2% fetal calf serum [PAN-Biotech, Aidenbach, Germany], 1% penicillin/streptomycin [Invitrogen], 1% L-glutamine [Invitrogen], 0.1 mM nonessential amino acids [Invitrogen], 10 mM HEPES [Invitrogen], 100 U/mL IL-4 [Peprotech], and 100 ng/mL granulocyte-macrophage colony-stimulating factor [Peprotech]) for 5 days.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Article Title: Anti-PD-1 antibodies and methods of use thereof
    Article Snippet: The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. The hu-PBMCs were washed 4 times with PBS and cluster of differentiation 14 (CD14+) monocytes were isolated using a human specific CD14 cell isolation kit with positive selection, as described in the manufacturer's protocol (Miltenyi Biotec, San Diego, Calif.). .. Cultures were supplemented with recombinant human (rh-) IL-4 (1000 U/mL) (R&D Systems, Minneapolis, Minn.) and with rh-granulocyte-macrophage colony-stimulating factor (GM-CSF) (rh-GMCSF) (500 U/mL) (R&D Systems, Minneapolis, Minn.) at Days 0, 2 and 5.

    Magnetic Cell Separation:

    Article Title: IL-36/LXR axis modulates cholesterol metabolism and immune defense to Mycobacterium tuberculosis
    Article Snippet: THP-1 monocytes (ATCC: TIB-202) were differentiated into macrophages using 100 nM PMA. .. For the generation of human MDM, peripheral blood CD14 + cells were positively selected by MACS using CD14 + cell isolation kit (Miltenyi Biotech, Bergisch Gladbach, Germany) according to manufacturer’s instructions and differentiated with GM-CSF at 20 ng/ml for 7 days in RPMI 1640. ..

    Generated:

    Article Title: Single-cell transcriptomics reveals prominent expression of IL-14, IL-18, and IL-32 in psoriasis.
    Article Snippet: In a final step, percentages for negative and 1+ to 3+ positive cells based on these representative slides were illustrated using RStudio (version 2022.07.2+576). .. Human DC were generated from CD14-positive monocytes from whole blood using a CD14+ cell isolation kit (Miltenyi) as described [50]. .. Cells were cultured in DC-medium (2% fetal calf serum [PAN-Biotech, Aidenbach, Germany], 1% penicillin/streptomycin [Invitrogen], 1% L-glutamine [Invitrogen], 0.1 mM nonessential amino acids [Invitrogen], 10 mM HEPES [Invitrogen], 100 U/mL IL-4 [Peprotech], and 100 ng/mL granulocyte-macrophage colony-stimulating factor [Peprotech]) for 5 days.



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    CD16 + macrophages constitute a high proportion in decidua with an immunoregulatory phenotype. (A) Expression of FCGR3A in immune cells from endometrium (Ctrl, n=3) or decidua (NP, n=3) in UMAP plots of scRNA-seq analysis. And the violin plots is shown in (B) . (C) Levels of CD16 in CD45 + <t>CD14</t> + macrophages from endometrium (n=10) or decidua (n=10) detected by flow cytometry assays. (D) Expression of CD86, HLA-DR, CD209 and CD206 in CD14 + CD16 + or CD14 + CD16 - dMφ (n=9) in flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001.
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    (A) Immunofluorescence images of live ZsGreen-expressing MCF7 tumorspheres alone or in co-culture with HuMoSC (1:5 ratio) after staining with <t>anti-CD14</t> (red) anti-Epcam (green) antibodies. Nuclei stained with Hoechst (blue). All images are representative of at least three replicates. White arrows points towards tumorspheres-associated HuMoSC. (B) Tumorsphere formation assay of MCF7 cells cultured alone, with CD52 + HuMoSC or with conditioned medium (CM) from HuMoSC (CM HuMoSC ) or from co-culture of MCF7 and HuMoSC (CM co-culture ). In some conditions, MCF7 were physically separated from HuMoSC cells by a transwell. Statistical significance was assessed using a Kruskal-Wallis test with Dunn’s multiple comparisons test. ***p<0.001; ns, not significant. Data are represented as mean values ± S.E.M. (C) CellChat inferred outgoing communication patterns of the different cell clusters (left panel) and associated signaling pathways (right panel). (D) Surfaceome workflow for the identification and quantification of total cell surface proteins. (E) Talkien-predicted protein interaction network of HuMoSC and MCF7 surfaceome. (F-G) Kernel density plot showing log2 intensity of TGFB1 in HuMoSC surfaceome (F) and TGF-BR1 (G) in MCF7 surfaceome. CD45 (F) and EPCAM (G) were used as reference for protein intensities. Dashed line: calculated standard deviation (SD) of the three replicates for the corresponding protein. (H) Chord diagram showing predicted TGFb signaling between clusters. Red arrows highlight the signaling specificity of CD52 + HuMoSC towards MCF7_1 and MCF7_2 clusters.
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    Image Search Results


    CD16 + macrophages constitute a high proportion in decidua with an immunoregulatory phenotype. (A) Expression of FCGR3A in immune cells from endometrium (Ctrl, n=3) or decidua (NP, n=3) in UMAP plots of scRNA-seq analysis. And the violin plots is shown in (B) . (C) Levels of CD16 in CD45 + CD14 + macrophages from endometrium (n=10) or decidua (n=10) detected by flow cytometry assays. (D) Expression of CD86, HLA-DR, CD209 and CD206 in CD14 + CD16 + or CD14 + CD16 - dMφ (n=9) in flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: CD16 + macrophages constitute a high proportion in decidua with an immunoregulatory phenotype. (A) Expression of FCGR3A in immune cells from endometrium (Ctrl, n=3) or decidua (NP, n=3) in UMAP plots of scRNA-seq analysis. And the violin plots is shown in (B) . (C) Levels of CD16 in CD45 + CD14 + macrophages from endometrium (n=10) or decidua (n=10) detected by flow cytometry assays. (D) Expression of CD86, HLA-DR, CD209 and CD206 in CD14 + CD16 + or CD14 + CD16 - dMφ (n=9) in flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: DMφ from DICs were obtained through positive selection by an CD14 + cell isolation kit according to the reagent instructions (Miltenyi Biotec, 130–050-201).

    Techniques: Expressing, Flow Cytometry, Two Tailed Test

    CD16 + macrophages from endometrium and decidua exhibit higher levels of adhesion molecules. (A) Expression of CD49a, CD44 and LYVE1 in CD45 + CD14 + CD16 + or CD45 + CD14 + CD16 - eMφ (n=12) in flow cytometry assays. (B) Expression of CD49a, CD44, LYVE1 and Integrin αVβ3 in CD14 + CD16 + or CD14 + CD16 - dMφ (n=11) in flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: CD16 + macrophages from endometrium and decidua exhibit higher levels of adhesion molecules. (A) Expression of CD49a, CD44 and LYVE1 in CD45 + CD14 + CD16 + or CD45 + CD14 + CD16 - eMφ (n=12) in flow cytometry assays. (B) Expression of CD49a, CD44, LYVE1 and Integrin αVβ3 in CD14 + CD16 + or CD14 + CD16 - dMφ (n=11) in flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns.

    Article Snippet: DMφ from DICs were obtained through positive selection by an CD14 + cell isolation kit according to the reagent instructions (Miltenyi Biotec, 130–050-201).

    Techniques: Expressing, Flow Cytometry, Two Tailed Test

    Patients RM exhibit weakened interactions between DSCs and CD16 + dMφ. (A) Compared the expression of COL4A1 in decidua from women with normal pregnancy (NP, n=8) or recurrent miscarriage (RM, n=8) by Immunohistochemistry. Scale bar, 50 μm. (B) Compared the expression of OPN in decidua from women with NP (n=10) or RM (n=10) by Immunohistochemistry. Scale bar, 50 μm. (C) Compared the expression of HA in decidua from women with NP (n=8) or RM (n=8) by Alcian blue staining assays. Scale bar, 50 μm. (D) Levels of CD16, CD86, CD206 and CD209 in CD45 + CD14 + dMφ from women with NP (n=7) or RM (n=7) by flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy

    doi: 10.3389/fimmu.2025.1747323

    Figure Lengend Snippet: Patients RM exhibit weakened interactions between DSCs and CD16 + dMφ. (A) Compared the expression of COL4A1 in decidua from women with normal pregnancy (NP, n=8) or recurrent miscarriage (RM, n=8) by Immunohistochemistry. Scale bar, 50 μm. (B) Compared the expression of OPN in decidua from women with NP (n=10) or RM (n=10) by Immunohistochemistry. Scale bar, 50 μm. (C) Compared the expression of HA in decidua from women with NP (n=8) or RM (n=8) by Alcian blue staining assays. Scale bar, 50 μm. (D) Levels of CD16, CD86, CD206 and CD209 in CD45 + CD14 + dMφ from women with NP (n=7) or RM (n=7) by flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: DMφ from DICs were obtained through positive selection by an CD14 + cell isolation kit according to the reagent instructions (Miltenyi Biotec, 130–050-201).

    Techniques: Expressing, Immunohistochemistry, Staining, Flow Cytometry, Two Tailed Test

    (A) Immunofluorescence images of live ZsGreen-expressing MCF7 tumorspheres alone or in co-culture with HuMoSC (1:5 ratio) after staining with anti-CD14 (red) anti-Epcam (green) antibodies. Nuclei stained with Hoechst (blue). All images are representative of at least three replicates. White arrows points towards tumorspheres-associated HuMoSC. (B) Tumorsphere formation assay of MCF7 cells cultured alone, with CD52 + HuMoSC or with conditioned medium (CM) from HuMoSC (CM HuMoSC ) or from co-culture of MCF7 and HuMoSC (CM co-culture ). In some conditions, MCF7 were physically separated from HuMoSC cells by a transwell. Statistical significance was assessed using a Kruskal-Wallis test with Dunn’s multiple comparisons test. ***p<0.001; ns, not significant. Data are represented as mean values ± S.E.M. (C) CellChat inferred outgoing communication patterns of the different cell clusters (left panel) and associated signaling pathways (right panel). (D) Surfaceome workflow for the identification and quantification of total cell surface proteins. (E) Talkien-predicted protein interaction network of HuMoSC and MCF7 surfaceome. (F-G) Kernel density plot showing log2 intensity of TGFB1 in HuMoSC surfaceome (F) and TGF-BR1 (G) in MCF7 surfaceome. CD45 (F) and EPCAM (G) were used as reference for protein intensities. Dashed line: calculated standard deviation (SD) of the three replicates for the corresponding protein. (H) Chord diagram showing predicted TGFb signaling between clusters. Red arrows highlight the signaling specificity of CD52 + HuMoSC towards MCF7_1 and MCF7_2 clusters.

    Journal: bioRxiv

    Article Title: Immunosuppressive myeloid cells induce mesenchymal-like breast cancer stem cells by a mechanism involving membrane-bound TGF-β1

    doi: 10.1101/2025.02.21.639264

    Figure Lengend Snippet: (A) Immunofluorescence images of live ZsGreen-expressing MCF7 tumorspheres alone or in co-culture with HuMoSC (1:5 ratio) after staining with anti-CD14 (red) anti-Epcam (green) antibodies. Nuclei stained with Hoechst (blue). All images are representative of at least three replicates. White arrows points towards tumorspheres-associated HuMoSC. (B) Tumorsphere formation assay of MCF7 cells cultured alone, with CD52 + HuMoSC or with conditioned medium (CM) from HuMoSC (CM HuMoSC ) or from co-culture of MCF7 and HuMoSC (CM co-culture ). In some conditions, MCF7 were physically separated from HuMoSC cells by a transwell. Statistical significance was assessed using a Kruskal-Wallis test with Dunn’s multiple comparisons test. ***p<0.001; ns, not significant. Data are represented as mean values ± S.E.M. (C) CellChat inferred outgoing communication patterns of the different cell clusters (left panel) and associated signaling pathways (right panel). (D) Surfaceome workflow for the identification and quantification of total cell surface proteins. (E) Talkien-predicted protein interaction network of HuMoSC and MCF7 surfaceome. (F-G) Kernel density plot showing log2 intensity of TGFB1 in HuMoSC surfaceome (F) and TGF-BR1 (G) in MCF7 surfaceome. CD45 (F) and EPCAM (G) were used as reference for protein intensities. Dashed line: calculated standard deviation (SD) of the three replicates for the corresponding protein. (H) Chord diagram showing predicted TGFb signaling between clusters. Red arrows highlight the signaling specificity of CD52 + HuMoSC towards MCF7_1 and MCF7_2 clusters.

    Article Snippet: Monocytes were then isolated from PBMC by magnetic cell sorting using the AutoMACS ® Pro Separator and Human CD14 + cell isolation kit (Miltenyi Biotec) following manufacturer’s indications.

    Techniques: Immunofluorescence, Expressing, Co-Culture Assay, Staining, Tube Formation Assay, Cell Culture, Protein-Protein interactions, Standard Deviation